Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR.

BMC biotechnology - 28 Aug 2009

Boyle Brian, Dallaire Nancy, MacKay John

Abstract excerpt

BACKGROUND: Robust designs of PCR-based molecular diagnostic assays rely on the discrimination potential of sequence variants affecting primer-to-template annealing. However, for accurate quantitative PCR (qPCR) assessment of gene expression in populations with gene polymorphisms, the effects of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to DNA, Complementary
Share to DNA, Plant

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Primers

Share to Expressed Sequence Tags

Share to Plasmids

Share to Polymerase Chain Reaction

Source

Open in PubMed Open full text

Identifiers

PMID: 19715565
DOI: 10.1186/1472-6750-9-75
PMCID: PMC2741440

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-19715565-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR.PMID 19715565Read ↗

Single-nucleotide polymorphisms and other mismatches reduce performance of quantitative PCR assays.Semantic proximity Read ↗

Computational tradeoffs in multiplex PCR assay design for SNP genotyping.Semantic proximity Read ↗

Predicting the success of primer extension genotyping assays using statistical modeling.Semantic proximity Read ↗

In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCRSemantic proximity Read ↗

In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR.Semantic proximity Read ↗

PrimerMapper: high throughput primer design and graphical assembly for PCR and SNP detection.Semantic proximity Read ↗

Tuning single nucleotide discrimination in polymerase chain reactions (PCRs): synthesis of primer probes bearing polar 4'-C-modifications and their application in allele-specific PCR.Semantic proximity Read ↗

Enhanced annealing of mismatched oligonucleotides using a novel melting curve assay allows efficient in vitro discrimination and restriction of a single nucleotide polymorphism.Semantic proximity Read ↗

Allele-Specific Mutation Genotyping with Mismatches in Primer Design.Semantic proximity Read ↗

Kinetic characterisation of primer mismatches in allele-specific PCR: a quantitative assessment.Semantic proximity Read ↗

Comparison of competitively primed and conventional allele-specific nucleic acid amplification.Semantic proximity Read ↗

Optimized detection of sequence variation in heterozygous genomes using DNA microarrays with isothermal-melting probes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.