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Article

Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR.

BMC biotechnology - 28 Aug 2009

Boyle Brian, Dallaire Nancy, MacKay John

Abstract excerpt

BACKGROUND: Robust designs of PCR-based molecular diagnostic assays rely on the discrimination potential of sequence variants affecting primer-to-template annealing. However, for accurate quantitative PCR (qPCR) assessment of gene expression in populations with gene polymorphisms, the effects of sequence variants within primer binding sites must be minimized. This dichotomy in PCR applications prompted us to...

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Identifiers

PMID: 19715565
DOI: 10.1186/1472-6750-9-75
PMCID: PMC2741440

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