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Article

In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR.

Journal of virological methods - 1 Jan 2022

Zimmermann Fee, Urban Maria, Krüger Christian, Walter Mathias, Wölfel Roman, Zwirglmaier Katrin

Abstract excerpt

A number of RT-qPCR assays for the detection of SARS-CoV-2 have been published and are listed by the WHO as recommended assays. Furthermore, numerous commercial assays with undisclosed primer and probe sequences are on the market. As the SARS-CoV-2 pandemic progresses, the virus accrues mutations, which in some cases - as seen with the B.1.1.7 variant - can outperform and push back other strains of SARS-CoV-2. If...

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Identifiers

PMID: 34748815
DOI: 10.1016/j.jviromet.2021.114352
PMCID: PMC8570391

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

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