Article
[Development of a real-time reverse transcriptase PCR assay for detection of E119V amino acid change in neuraminidase of influenza A (H3N2) using the TaqMan-MGB probe].
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] - 1 May 2013
Zhao Xiang, Huang Wei-juan, Wei He-jiang, Wang Zhao, Li Xi-yan, Cheng Yan-hui, Tan Min-ju, Xiao Ning, Lan Yu, Guo Jun-feng, Sui Hong-tao, Zhu Wen-fei, Du Dong-dong, Wang Da-yan, Shu Yue-long
Abstract excerpt
OBJECTIVE: To develop a rapid duplex Real-time reverse transcription PCR (rRT-PCR) method to detect E119V mutation on neuraminidase (NA) of influenza A(H3N2) subtype with drug resistance to oseltamivir. METHODS: Twenty-six NA genes of influenza A(H3N2) virus between 2000 and 2012 in GenBank datab...
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