Article
The impact of primer and probe-template mismatches on the sensitivity of pandemic influenza A/H1N1/2009 virus detection by real-time RT-PCR.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Jun 2010
Klungthong Chonticha, Chinnawirotpisan Piyawan, Hussem Kittinun, Phonpakobsin Thipwipha, Manasatienkij Wudtichai, Ajariyakhajorn Chuanpis, Rungrojcharoenkit Kamonthip, Gibbons Robert V, Jarman Richard G
Abstract excerpt
BACKGROUND: In response to the 2009 H1N1 pandemic the US CDC and WHO rapidly developed and distributed a real-time RT-PCR kit to detect this strain in clinical samples. The results from the WHO swH1 primer and probe set exhibited diverse sensitivities for the 2009 influenza A/H1N1 strains in Southeast Asia (SEA). OBJECTIVE: Investigate the primer and probe-template mismatches among the 2009 influenza A/H1N1...
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