Article
Molecular assays for quantitative and qualitative detection of influenza virus and oseltamivir resistance mutations.
The Journal of molecular diagnostics : JMD - 1 May 2013
van der Vries Erhard, Anber Jeer, van der Linden Anne, Wu Yingbin, Maaskant Jolanda, Stadhouders Ralph, van Beek Ruud, Rimmelzwaan Guus, Osterhaus Albert, Boucher Charles, Schutten Martin
Abstract excerpt
Sensitive and reproducible molecular assays are essential for influenza virus diagnostics. This manuscript describes the design, validation, and evaluation of a set of real-time RT-PCR assays for quantification and subtyping of human influenza viruses from patient respiratory material. Four assays are included for detection of oseltamivir resistance mutations H275Y in prepandemic and pandemic influenza A/H1N1 and...
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