Article
Coupling an universal primer to SBE combined spectral codification strategy for single nucleotide polymorphism analysis.
Journal of biotechnology - 10 Oct 2013
Cordeiro Mílton, Giestas Letícia, Lima João C, Baptista Pedro
Abstract excerpt
We previously reported a strategy that combines Förster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an...
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