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Article

Fluorescence-based DHPLC for allelic quantification by single-nucleotide primer extension.

Journal of biochemical and biophysical methods - 30 Jan 2001

Kosaki K, Yoshihashi H, Ohashi Y, Kosaki R, Suzuki T, Matsuo N

Abstract excerpt

We have investigated the possibility of determining quantitatively the alleles of binary DNA polymorphisms by single-nucleotide primer extension (SNuPE) and fluorescence-based DHPLC. Using a polymorphism of interest to our group, ROX-labeled dideoxy CTP (ROX-ddCTP) was incorporated at the 3' end of the primer annealed to the template adjacent to the polymorphic site. The primer extension product was then resolved...

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PMID: 11179767
DOI: 10.1016/s0165-022x(00)00157-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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