Article
Nanofluidic digital PCR for KRAS mutation detection and quantification in gastrointestinal cancer.
Clinical chemistry - 1 Sept 2012
Azuara Daniel, Ginesta Mireia M, Gausachs Mireia, Rodriguez-Moranta Francisco, Fabregat Joan, Busquets Juli, Pelaez Nuria, Boadas Jaume, Galter Sara, Moreno Victor, Costa Jose, de Oca Javier, Capellá Gabriel
Abstract excerpt
BACKGROUND: Concomitant quantification of multiple mutant KRAS (v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog) alleles may provide information in addition to that provided by standard mutation-detection procedures. We assessed the feasibility of a nanofluidic digital PCR array platform to detect and quantify KRAS mutations simultaneously in clinically relevant samples. METHODS: We assessed 2 groups of...
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