Article
Construction, characterization, and utilization of cell lines which inducibly express the adenovirus DNA-binding protein.
Virology - 1 Oct 1992
Brough D E, Cleghon V, Klessig D F
Abstract excerpt
To further our understanding of structure-function relationships within the multifunctional adenovirus DNA binding protein (DBP) a more diverse collection of mutants is necessary. DBP-expressing cell lines (gmDBP) were previously constructed that complemented DBP-negative mutants for viral growth. However, they did not allow severely defective viruses to form plaques. Since efficient mutant construction is...
Topics
- Adenovirus E2 Proteins
- Cloning, Molecular
- DNA Replication
- DNA, Viral
- DNA-Binding Proteins
- Dexamethasone
- Gene Expression Regulation, Viral
- HeLa Cells
- Humans
- Mutation
- Plasmids