Article
Purification and functional characterization of adenovirus ts111A DNA-binding protein. Fluorescence studies of protein-nucleic acid binding.
The Journal of biological chemistry - 5 Apr 1990
Meyers M L, Keating K M, Roberts W J, Williams K R, Chase J W, Horwitz M S
Abstract excerpt
The adenovirus single-stranded DNA (ssDNA)-binding protein (DBP) is necessary for the elongation step in viral DNA replication. In an attempt to characterize the putative ssDNA-binding domain of the DBP, we purified and characterized the Ad2ts111A DBP, which contains a glycine-to-valine substitution at amino acid 280. This mutation is adjacent to that in the previously studied Ad2+ND1ts23. Ad2+ND1ts23 exhibits a...
Topics
- Adenoviridae
- Binding Sites
- Chymotrypsin
- DNA Replication
- DNA, Single-Stranded
- DNA, Viral
- DNA-Binding Proteins
- HeLa Cells
- Humans
- Mutation
- Nucleic Acids