Article
Tag-extension-based method for sensitive and specific genotyping of single nucleotide polymorphism on microarray.
Clinica chimica acta; international journal of clinical chemistry - 1 Nov 2009
Shan Qun, Zheng Yuanlin, Chen Guoqing, Zheng Guihong, Lu Jun, Lv Xiaojuan
Abstract excerpt
BACKGROUND: The obtainment of a large amount of single nucleotide polymorphism (SNP) information has emphasized a need for a sensitive, accurate and high-throughput strategy for SNP genotyping. We developed a reliable and potential microarray-based method to meet this demand. METHODS: A tag extension strategy is described to identify SNPs. The strategy is based on a fluorescent nucleotide extension from an...
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