Article
Structural analysis of H-2Kf and H-2Kfm1 by using H-2K locus-specific sequences.
Journal of immunology (Baltimore, Md. : 1950) - 15 Sept 1990
Horton R M, Hildebrand W H, Martinko J M, Pease L R
Abstract excerpt
The H-2Kf allele and the spontaneous mutant Kfm1 have been cloned using locus-specific sequences. The mutation consists of a cluster of four nucleotide changes, resulting in amino acid substitutions at positions 95 (Leu----Ile) and 97 (Val----Arg). This finding has structural, genetic, and technical implications. The amino acid substitutions are located on the beta-strands of the antigen recognition site. Their...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Base Sequence
- Exons
- H-2 Antigens
- Mice
- Mice, Inbred Strains
- Molecular Sequence Data
- Mutation
- Oligonucleotides
- Polymerase Chain Reaction