Article
Pooled deep sequencing of Plasmodium falciparum isolates: an efficient and scalable tool to quantify prevailing malaria drug-resistance genotypes.
The Journal of infectious diseases - 15 Dec 2013
Taylor Steve M, Parobek Christian M, Aragam Nash, Ngasala Billy E, Mårtensson Andreas, Meshnick Steven R, Juliano Jonathan J
Abstract excerpt
Molecular surveillance for drug-resistant malaria parasites requires reliable, timely, and scalable methods. These data may be efficiently produced by genotyping parasite populations using second-generation sequencing (SGS). We designed and validated a SGS protocol to quantify mutant allele frequencies in the Plasmodium falciparum genes dhfr and dhps in mixed isolates. We applied this new protocol to field...
Topics
- Antimalarials
- Base Sequence
- Child
- DNA, Protozoan
- Drug Resistance
- Gene Frequency
- Genotype
- High-Throughput Nucleotide Sequencing
- Humans
- Malaria, Falciparum
- Molecular Epidemiology
- Molecular Sequence Data
- Plasmodium falciparum