Article
Analysis of Plasmodium falciparum diversity in natural infections by deep sequencing.
Nature - 19 Jul 2012
Manske Magnus, Miotto Olivo, Campino Susana, Auburn Sarah, Almagro-Garcia Jacob, Maslen Gareth, O'Brien Jack, Djimde Abdoulaye, Doumbo Ogobara, Zongo Issaka, Ouedraogo Jean-Bosco, Michon Pascal, Mueller Ivo, Siba Peter, Nzila Alexis, Borrmann Steffen, Kiara Steven M, Marsh Kevin, Jiang Hongying, Su Xin-Zhuan, Amaratunga Chanaki, Fairhurst Rick, Socheat Duong, Nosten Francois, Imwong Mallika, White Nicholas J, Sanders Mandy, Anastasi Elisa, Alcock Dan, Drury Eleanor, Oyola Samuel, Quail Michael A, Turner Daniel J, Ruano-Rubio Valentin, Jyothi Dushyanth, Amenga-Etego Lucas, Hubbart Christina, Jeffreys Anna, Rowlands Kate, Sutherland Colin, Roper Cally, Mangano Valentina, Modiano David, Tan John C, Ferdig Michael T, Amambua-Ngwa Alfred, Conway David J, Takala-Harrison Shannon, Plowe Christopher V, Rayner Julian C, Rockett Kirk A, Clark Taane G, Newbold Chris I, Berriman Matthew, MacInnis Bronwyn, Kwiatkowski Dominic P
Abstract excerpt
Malaria elimination strategies require surveillance of the parasite population for genetic changes that demand a public health response, such as new forms of drug resistance. Here we describe methods for the large-scale analysis of genetic variation in Plasmodium falciparum by deep sequencing of parasite DNA obtained from the blood of patients with malaria, either directly or after short-term culture. Analysis of...
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