Article
Gauging NOTCH1 Activation in Cancer Using Immunohistochemistry.
PloS one - 1 Jan 2013
Kluk Michael J, Ashworth Todd, Wang Hongfang, Knoechel Birgit, Mason Emily F, Morgan Elizabeth A, Dorfman David, Pinkus Geraldine, Weigert Oliver, Hornick Jason L, Chirieac Lucian R, Hirsch Michelle, Oh David J, South Andrew P, Leigh Irene M, Pourreyron Celine, Cassidy Andrew J, Deangelo Daniel J, Weinstock David M, Krop Ian E, Dillon Deborah, Brock Jane E, Lazar Alexander J F, Peto Myron, Cho Raymond J, Stoeck Alexander, Haines Brian B, Sathayanrayanan Sriram, Rodig Scott, Aster Jon C
Abstract excerpt
Fixed, paraffin-embedded (FPE) tissues are a potentially rich resource for studying the role of NOTCH1 in cancer and other pathologies, but tests that reliably detect activated NOTCH1 (NICD1) in FPE samples have been lacking. Here, we bridge this gap by developing an immunohistochemical (IHC) stain that detects a neoepitope created by the proteolytic cleavage event that activates NOTCH1. Following validation...
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