Article
AutoCloner: automatic homologue-specific primer design for full-gene cloning in polyploids.
BMC bioinformatics - 16 Jul 2020
Coulton Alexander, Edwards Keith J
Abstract excerpt
BACKGROUND: Polyploid organisms such as wheat complicate even the simplest of procedures in molecular biology. Whilst knowledge of genomic sequences in crops is increasing rapidly, the scientific community is still a long way from producing a full pan-genome for every species. Polymerase chain reaction and Sanger sequencing therefore remain widely used as methods for characterizing gene sequences in many...
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