Article
Enrichment and detection of rare alleles by means of snapback primers and rapid-cycle PCR.
Clinical chemistry - 1 May 2010
Zhou Luming, Palais Robert A, Smith G Denice, Anderson Daniel, Rowe Leslie R, Wittwer Carl T
Abstract excerpt
BACKGROUND: Selective amplification of minority alleles is often necessary to detect cancer mutations in clinical samples. METHODS: Minor-allele enrichment and detection were performed with snapback primers in the presence of a saturating DNA dye within a closed tube. A 5' tail of nucleotides on 1 PCR primer hybridizes to the variable locus of its extension product to produce a hairpin that selectively enriches...
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