Article
O6-methylguanine and A.C and G.T mismatches cause asymmetric structural defects in DNA that are affected by DNA sequence.
Biochemistry - 29 May 1990
Voigt J M, Topal M D
Abstract excerpt
Mismatched and modified base pairs are central to questions of DNA mutation and repair. NMR and X-ray crystallography of mispairs indicate little to no local helical distortion, but these techniques are not sensitive to more global distortions of the DNA molecule. We used polyacrylamide gel electrophoresis and thermal denaturation to examine A.C, G.T, and O6-methylG.T and O6-methylG.C mismatches synthesized in...
Topics
- Base Composition
- Base Sequence
- DNA, Viral
- Electrophoresis, Polyacrylamide Gel
- Guanine
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Nucleic Acid Denaturation
- T-Phages
- Temperature