Article
User guide for mapping-by-sequencing in Arabidopsis.
Genome biology - 17 Jun 2013
James Geo Velikkakam, Patel Vipul, Nordström Karl J V, Klasen Jonas R, Salomé Patrice A, Weigel Detlef, Schneeberger Korbinian
Abstract excerpt
Mapping-by-sequencing combines genetic mapping with whole-genome sequencing in order to accelerate mutant identification. However, application of mapping-by-sequencing requires decisions on various practical settings on the experimental design that are not intuitively answered. Following an experimentally determined recombination landscape of Arabidopsis and next generation sequencing-specific biases, we...
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