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CTAB DNA Extraction and Genotyping-by-Sequencing to Map Meiotic Crossovers in Plants.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022

Kuo Pallas, Henderson Ian R, Lambing Christophe

Abstract excerpt

Reciprocal DNA crossovers between chromosomes form new allelic combinations and contribute to the formation of novel genetic diversity. Crossovers are formed during meiosis of germ cells and these recombination events have influenced plant genome evolution and are used during breeding to create improved plant varieties. Meiotic crossovers are not uniformly formed across the genome but instead occur in regions...

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PMID: 35461443
DOI: 10.1007/978-1-0716-2253-7_4

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