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Article

Molecular dissection of human Argonaute proteins by DNA shuffling.

Nature structural & molecular biology - 1 Jul 2013

Schürmann Nina, Trabuco Leonardo G, Bender Christian, Russell Robert B, Grimm Dirk

Abstract excerpt

A paramount task in RNA interference research is to decipher the complex biology of cellular effectors, exemplified in humans by four pleiotropic Argonaute proteins (Ago1-Ago4). Here, we exploited DNA family shuffling, a molecular evolution technology, to generate chimeric Ago protein libraries for dissection of intricate phenotypes independently of prior structural knowledge. Through shuffling of human Ago2 and...

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Identifiers

PMID: 23748378
DOI: 10.1038/nsmb.2607

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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