Article
T-cell receptor specificity maintained by altered thermodynamics.
The Journal of biological chemistry - 28 Jun 2013
Madura Florian, Rizkallah Pierre J, Miles Kim M, Holland Christopher J, Bulek Anna M, Fuller Anna, Schauenburg Andrea J A, Miles John J, Liddy Nathaniel, Sami Malkit, Li Yi, Hossain Moushumi, Baker Brian M, Jakobsen Bent K, Sewell Andrew K, Cole David K
Abstract excerpt
The T-cell receptor (TCR) recognizes peptides bound to major histocompatibility molecules (MHC) and allows T-cells to interrogate the cellular proteome for internal anomalies from the cell surface. The TCR contacts both MHC and peptide in an interaction characterized by weak affinity (KD = 100 nM to 270 μM). We used phage-display to produce a melanoma-specific TCR (α24β17) with a 30,000-fold enhanced binding...
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