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Article

Kinetic analysis of inhibition of glucoamylase and active site mutants via chemoselective oxime immobilization of acarbose on SPR chip surfaces.

Carbohydrate research - 28 Jun 2013

Sauer Jørgen, Abou Hachem Maher, Svensson Birte, Jensen Knud J, Thygesen Mikkel B

Abstract excerpt

We here report a quantitative study on the binding kinetics of inhibition of the enzyme glucoamylase and how individual active site amino acid mutations influence kinetics. To address this challenge, we have developed a fast and efficient method for anchoring native acarbose to gold chip surfaces for surface plasmon resonance studies employing wild type glucoamylase and active site mutants, Y175F, E180Q, and...

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PMID: 23680647
DOI: 10.1016/j.carres.2013.04.012

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SPR

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