Article
Peptide biosensors for the electrochemical measurement of protein kinase activity.
Analytical chemistry - 15 Dec 2008
Kerman Kagan, Song Haifeng, Duncan James S, Litchfield David W, Kraatz Heinz-Bernhard
Abstract excerpt
The kinase activities are elucidated using the novel redox-active cosubstrate adenosine 5'-[gamma-ferrocene] triphosphate (Fc-ATP), which enables the kinase-catalyzed transfer of a redox active gamma-phosphate-Fc to a hydroxyamino acid. In this report, a versatile electrochemical biosensor is developed for monitoring the activity and inhibition of a serine/threonine kinase, casein kinase 2 (CK2), and protein...
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