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Article

Both binding sites of the starch-binding domain of Aspergillus niger glucoamylase are essential for inducing a conformational change in amylose.

Journal of molecular biology - 9 Nov 2001

Giardina T, Gunning A P, Juge N, Faulds C B, Furniss C S, Svensson B, Morris V J, Williamson G

Abstract excerpt

The interaction of the two binding sites of the starch-binding domain (SBD) of Aspergillus niger glucoamylase 1 (GA-I) with substrate has been investigated by using atomic force microscopy (AFM) and UV difference spectroscopy in combination with site-specific mutants of both SBD and GA-I. The SBD possesses two binding sites with distinct affinities towards the soluble linear substrate maltoheptaose; dissociation...

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Source

Identifiers

PMID: 11700070
DOI: 10.1006/jmbi.2001.5097

Genes

AFM

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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