Article
Digital quantitation of potential therapeutic target RNAs.
Nucleic acid therapeutics - 1 Jun 2013
Dodd David W, Gagnon Keith T, Corey David R
Abstract excerpt
Accurate determination of the amount of a given RNA within a cell is necessary to gain a full understanding of the RNA's function and regulation. Typically, the abundance of RNA is measured by quantitative polymerase chain reaction (qPCR). With qPCR, however, absolute quantification is not possible unless an adequate reference standard curve is generated. The method is not well suited for detecting low copy...
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