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Article

A genetic screen using the PiggyBac transposon in haploid cells identifies Parp1 as a mediator of olaparib toxicity.

PloS one - 1 Jan 2013

Pettitt Stephen J, Rehman Farah L, Bajrami Ilirjana, Brough Rachel, Wallberg Fredrik, Kozarewa Iwanka, Fenwick Kerry, Assiotis Ioannis, Chen Lina, Campbell James, Lord Christopher J, Ashworth Alan

Abstract excerpt

Genetic perturbation screens have the potential to dissect a wide range of cellular phenotypes. Such screens have historically been difficult in diploid mammalian cells. The recent derivation of haploid embryonic stem cells provides an opportunity to cause loss of function mutants with a random mutagen in a mammalian cell with a normal genetic background. We describe an approach to genetic screens that exploits...

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Identifiers

PMID: 23634208
DOI: 10.1371/journal.pone.0061520
PMCID: PMC3636235

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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