Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Reorganization of an intersubunit bridge induced by disparate 16S ribosomal ambiguity mutations mimics an EF-Tu-bound state.

Proceedings of the National Academy of Sciences of the United States of America - 11 Jun 2013

Fagan Crystal E, Dunkle Jack A, Maehigashi Tatsuya, Dang Mai N, Devaraj Aishwarya, Miles Stacey J, Qin Daoming, Fredrick Kurt, Dunham Christine M

Abstract excerpt

After four decades of research aimed at understanding tRNA selection on the ribosome, the mechanism by which ribosomal ambiguity (ram) mutations promote miscoding remains unclear. Here, we present two X-ray crystal structures of the Thermus thermophilus 70S ribosome containing 16S rRNA ram mutati...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Crystallography, X-Ray
Share to Guanosine Triphosphate

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Hydrolysis

Share to Molecular Conformation

Share to Nucleic Acid Conformation

Share to Peptide Elongation Factor Tu

Share to RNA, Transfer

Share to Thermus thermophilus

Source

Open in PubMed Open full text

Identifiers

PMID: 23630274
DOI: 10.1073/pnas.1301585110
PMCID: PMC3683721

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-23630274-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Reorganization of an intersubunit bridge induced by disparate 16S ribosomal ambiguity mutations mimics an EF-Tu-bound state.PMID 23630274Read ↗

Ribosomal ambiguity (ram) mutations promote the open (off) to closed (on) transition and thereby increase miscoding.Semantic proximity Read ↗

Distinct functional classes of ram mutations in 16S rRNA.Semantic proximity Read ↗

Epistasis analysis of 16S rRNA ram mutations helps define the conformational dynamics of the ribosome that influence decoding.Semantic proximity Read ↗

Mutations in the intersubunit bridge regions of 16S rRNA affect decoding and subunit-subunit interactions on the 70S ribosome.Semantic proximity Read ↗

Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.Semantic proximity Read ↗

How mutations in tRNA distant from the anticodon affect the fidelity of decoding.Semantic proximity Read ↗

An arc of unpaired "hinge bases" facilitates information exchange among functional centers of the ribosome.Semantic proximity Read ↗

Disruption of evolutionarily correlated tRNA elements impairs accurate decoding.Semantic proximity Read ↗

Alterations in ribosomal protein L19 that decrease the fidelity of translation.Semantic proximity Read ↗

Diverse effects of residues 74-78 in ribosomal protein S12 on decoding and antibiotic sensitivity.Semantic proximity Read ↗

Hyperaccurate and error-prone ribosomes exploit distinct mechanisms during tRNA selection.Semantic proximity Read ↗

Interaction between 25S rRNA A loop and eukaryotic translation initiation factor 5B promotes subunit joining and ensures stringent AUG selection.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.