Article
cis-acting sequences and trans-acting factors required for constitutive expression of a microinjected HSP70 gene after the midblastula transition of Xenopus laevis embryogenesis.
Developmental genetics - 1 Jan 1990
Ovsenek N, Williams G T, Morimoto R I, Heikkila J J
Abstract excerpt
Microinjected human HSP70 promoter-chloramphenical acetyl transferase (CAT) chimeric genes are constitutively expressed immediately after the midblastula transition of Xenopus embryogenesis. Analysis of a series of 5'-deletion mutants in the HSP70 promoter revealed that sequences within 74 bases...
Topics
- Animals
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- DNA
- Gene Expression Regulation
- Heat-Shock Proteins
- Humans
- Microinjections
- Mutation
- Promoter Regions, Genetic
- Trans-Activators
- Xenopus laevis
