UV cross-linking identifies four polypeptides that require the TATA box to bind to the Drosophila hsp70 promoter.

Abstract excerpt

A protein fraction that requires the TATA sequence to bind to the hsp70 promoter has been partially purified from nuclear extracts of Drosophila embryos. This TATA factor produces a large DNase I footprint that extends from -44 to +35 on the promoter. A mutation that changes TATA to TATG interferes both with the binding of this complex and with the transcription of the hsp70 promoter in vitro, indicating that...

Topics

• Animals
• Base Sequence
• Binding Sites
• Cell Nucleus
• Cross-Linking Reagents
• Deoxyribonuclease I
• Drosophila
• Embryo, Nonmammalian
• Heat-Shock Proteins
• Macromolecular Substances
• Molecular Sequence Data