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Article

Induction of extremely low protein expression level by fusion of C-terminal region of Nef.

Biotechnology and applied biochemistry - 1 Jan 2000

Takamune Nobutoki, Irisaka Yukari, Yamamoto Minami, Harada Keisuke, Shoji Shozo, Misumi Shogo

Abstract excerpt

Nef is one of the accessory proteins of human immunodeficiency viruses. Here, we noted that the relative expression level of Nef(NL4-3) is much lower than that of NefJR-CSF in HEK293 cells. By evaluating the expression level using a Nef mutant, it was indicated that amino acids 129-206 of Nef(NL4-3), that is, the C-terminal region named NLAA129-206, could contain the region responsible for the induction of the...

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PMID: 23586835
DOI: 10.1002/bab.1021

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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