Article
An efficient positive selection procedure for the isolation of peroxisomal import and peroxisome assembly mutants of Saccharomyces cerevisiae.
Genetics - 1 Nov 1993
Elgersma Y, van den Berg M, Tabak H F, Distel B
Abstract excerpt
To study peroxisome biogenesis, we developed a procedure to select for Saccharomyces cerevisiae mutants defective in peroxisomal protein import or peroxisome assembly. For this purpose, a chimeric gene was constructed encoding the bleomycin resistance protein linked to the peroxisomal protein luc...
Topics
- Bleomycin
- Drug Resistance, Microbial
- Fungal Proteins
- Genes, Fungal
- Genetic Complementation Test
- Genetic Techniques
- Luciferases
- Microbodies
- Mutation
- Phleomycins
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae