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Article

Novel method for PIK3CA mutation analysis: locked nucleic acid--PCR sequencing.

The Journal of molecular diagnostics : JMD - 1 May 2013

Ang Daphne, O'Gara Rebecca, Schilling Amy, Beadling Carol, Warrick Andrea, Troxell Megan L, Corless Christopher L

Abstract excerpt

Somatic mutations in PIK3CA are commonly seen in invasive breast cancer and several other carcinomas, occurring in three hotspots: codons 542 and 545 of exon 9 and in codon 1047 of exon 20. We designed a locked nucleic acid (LNA)-PCR sequencing assay to detect low levels of mutant PIK3CA DNA with attention to avoiding amplification of a pseudogene on chromosome 22 that has >95% homology to exon 9 of PIK3CA. We...

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Identifiers

PMID: 23541593
DOI: 10.1016/j.jmoldx.2012.12.005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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