Article
A generic, whole-cell-based screening method for Baeyer-Villiger monooxygenases.
Journal of biomolecular screening - 1 Jul 2013
Dudek Hanna M, Popken Petra, van Bloois Edwin, Duetz Wouter A, Fraaije Marco W
Abstract excerpt
Baeyer-Villiger monooxygenases (BVMOs) have been receiving increasing attention as enzymes useful for biocatalytic applications. Industrial requirements call for rapid and extensive redesign of these enzymes. In response to the need for screening large libraries of BVMO mutants, we established a generic screening method that allows screening of Escherichia coli cells expressing active BVMOs in 96-well plate...
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