Article
Intra- and inter-molecular effects of a conserved arginine residue of neuronal and inducible nitric oxide synthases on FMN and calmodulin binding.
Archives of biochemistry and biophysics - 1 May 2013
Panda Satya Prakash, Polusani Srikanth R, Kellogg Dean L, Venkatakrishnan Priya, Roman Madeline G, Demeler Borries, Masters Bettie Sue S, Roman Linda J
Abstract excerpt
Nitric oxide synthases (NOSs) synthesize nitric oxide (NO), a signaling molecule, from l-arginine, utilizing electrons from NADPH. NOSs are flavo-hemo proteins, with two flavin molecules (FAD and FMN) and one heme per monomer, which require the binding of calcium/calmodulin (Ca(2+)/CaM) to produce NO. It is therefore important to understand the molecular factors influencing CaM binding from a structure/function...
Topics
- Animals
- Arginine
- Calmodulin
- Cell Line
- Conserved Sequence
- Electron Transport
- Flavin Mononucleotide
- Kinetics
- Mice
- Mutation
- Nitric Oxide Synthase Type I
- Nitric Oxide Synthase Type II
- Protein Binding