Article
Differential binding of calmodulin domains to constitutive and inducible nitric oxide synthase enzymes.
Biochemistry - 17 Jul 2007
Spratt Donald E, Taiakina Valentina, Palmer Michael, Guillemette J Guy
Abstract excerpt
Calmodulin (CaM) is a Ca2+ signal transducing protein that binds and activates many cellular enzymes with physiological relevance, including the mammalian nitric oxide synthase (NOS) isozymes: endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS). The mechanism of CaM binding and activation to the iNOS enzyme is poorly understood in part due to the strength of the bound complex and the difficulty...
Topics
- Binding Sites
- Calcium
- Calmodulin
- Cysteine
- Fluorescence Resonance Energy Transfer
- Fluorescent Dyes
- Holoenzymes
- Mutation
- Nitric Oxide Synthase Type I
- Nitric Oxide Synthase Type II
- Nitric Oxide Synthase Type III
- Peptides
- Protein Structure, Secondary
- Protein Structure, Tertiary
- Quinolinium Compounds