Article
Binding and activation of nitric oxide synthase isozymes by calmodulin EF hand pairs.
The FEBS journal - 1 Apr 2006
Spratt Donald E, Newman Elena, Mosher Jennifer, Ghosh Dipak K, Salerno John C, Guillemette J G
Abstract excerpt
Calmodulin (CaM) is a cytosolic Ca(2+) signal-transducing protein that binds and activates many different cellular enzymes with physiological relevance, including the nitric oxide synthase (NOS) isozymes. CaM consists of two globular domains joined by a central linker; each domain contains an EF hand pair. Four different mutant CaM proteins were used to investigate the role of the two CaM EF hand pairs in the...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- Calmodulin
- Cattle
- EF Hand Motifs
- Electron Transport
- Electrophoretic Mobility Shift Assay
- Enzyme Activation
- Escherichia coli
- Humans
- Isoenzymes
- Molecular Sequence Data
- Mutation