Article
A peptide nucleic acid (PNA) heteroduplex probe containing an inosine-cytosine base pair discriminates a single-nucleotide difference in RNA.
Chemistry (Weinheim an der Bergstrasse, Germany) - 15 Apr 2013
Matsumoto Katsuhiko, Nakata Eiji, Tamura Tomoki, Saito Isao, Aizawa Yasunori, Morii Takashi
Abstract excerpt
Selective discrimination of a single-nucleotide difference in single-stranded DNA or RNA remains a challenge with conventional DNA or RNA probes. A peptide nucleic acid (PNA)-derived probe, in which PNA forms a pseudocomplementary heteroduplex with inosine-containing DNA or RNA, effectively discriminates a single-nucleotide difference in a closely related group of sequences of single-stranded DNA and/or RNA. The...
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