Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

P450 oxidoreductase: genotyping, expression, purification of recombinant protein, and activity assessments of wild-type and mutant protein.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Agrawal Vishal, Miller Walter L

Abstract excerpt

P450 oxidoreductase (POR) is the flavoprotein that transfers electrons from NADPH to microsomal cytochrome P450 enzymes and to some other proteins. Protocols for genotyping human POR for common polymorphisms are described. Expression in E. coli of recombinant human POR, its purification, and different methods of assessing the effect of amino-acid sequence variants of POR on the activity of various cytochromes...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Membrane
Share to Enzyme Assays
Share to Escherichia coli

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genotyping Techniques

Share to Microsomes

Share to NADPH-Ferrihemoprotein Reductase

Share to Nickel

Share to Recombinant Proteins

Source

Identifiers

PMID: 23475681
DOI: 10.1007/978-1-62703-321-3_19

Genes

POR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

P450 oxidoreductase: genotyping, expression, purification of recombinant protein, and activity assessments of wild-type and mutant protein.PMID 23475681Read ↗

NADPH P450 oxidoreductase: structure, function, and pathology of diseases.Semantic proximity Read ↗

Exploring Novel Variants of Cytochrome P450 Reductase Gene (POR) from the Genome Aggregation Database by Integrating Bioinformatic Tools and Functional AssaysSemantic proximity Read ↗

Pharmacogenetics of P450 oxidoreductase: effect of sequence variants on activities of CYP1A2 and CYP2C19.Semantic proximity Read ↗

Exploring Novel Variants of the Cytochrome P450 Reductase Gene (POR) from the Genome Aggregation Database by Integrating Bioinformatic Tools and Functional Assays.Semantic proximity Read ↗

Loss of multiple enzyme activities due to the human genetic variation P284T in NADPH cytochrome P450 oxidoreductaseSemantic proximity Read ↗

Genetic polymorphisms in cytochrome P450 oxidoreductase influence microsomal P450-catalyzed drug metabolism.Semantic proximity Read ↗

Biochemical analysis of mutations in P450 oxidoreductase.Semantic proximity Read ↗

Variability in human drug metabolizing cytochrome P450 CYP2C9, CYP2C19 and CYP3A5 activities caused by genetic variations in cytochrome P450 oxidoreductaseSemantic proximity Read ↗

Clinical, structural and functional implications of mutations and polymorphisms in human NADPH P450 oxidoreductase.Semantic proximity Read ↗

Polymorphisms in cytochrome P450 oxidoreductase and its effect on drug metabolism and efficacy.Semantic proximity Read ↗

Variability in Loss of Multiple Enzyme Activities Due to the Human Genetic Variation P284T Located in the Flexible Hinge Region of NADPH Cytochrome P450 OxidoreductaseSemantic proximity Read ↗

Genetics of P450 oxidoreductase: sequence variation in 842 individuals of four ethnicities and activities of 15 missense mutations.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.