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Article

Asp48 function in the hydrogen-bonding network involving Asp52 of hen egg-white lysozyme.

Bioscience, biotechnology, and biochemistry - 1 Jan 2015

Kawaguchi Yuya, Yoneda Kazunari, Torikata Takao, Araki Tomohiro

Abstract excerpt

To characterize the hydrogen-bonding network in lysozyme, we focused on the residue of Asp48 located at the active site in hen egg-white lysozyme. We constructed a mutant lysozyme (D48A) and analyzed using (GlcNAc)3 and chitin-affinity chromatography. The substrate binding of subsites D-F in D48A and the activity against (GlcNAc)5 were decreased. The parameters of substrate binding and rate constants obtained...

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Identifiers

PMID: 25514638
DOI: 10.1080/09168451.2014.963502

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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