Article
Effect of cavity-modulating mutations on the stability of Escherichia coli ribonuclease HI.
European journal of biochemistry - 1 Jun 1992
Kimura S, Oda Y, Nakai T, Katayanagi K, Kitakuni E, Nakai C, Nakamura H, Ikehara M, Kanaya S
Abstract excerpt
The size of the cavity around Ser68 of Escherichia coli ribonuclease HI was modulated by amino acid substitutions to examine the effects on the stability of the enzyme. Five mutant proteins, Ser68----Gly, Ser68----Ala, Ser68----Thr, Ser68----Val and Ser68----Leu, were constructed. Each of the mut...
Topics
- Base Sequence
- Circular Dichroism
- DNA, Bacterial
- Enzyme Stability
- Escherichia coli
- Hot Temperature
- Isoenzymes
- Magnetic Resonance Spectroscopy
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Protein Denaturation
- Ribonuclease H
- Urea