Effects of subsite alterations on substrate-binding mode in the active site of hen egg-white lysozyme.

doi:10.1111/j.1432-1033.1993.tb17645.x

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Article

Effects of subsite alterations on substrate-binding mode in the active site of hen egg-white lysozyme.

European journal of biochemistry - 15 Feb 1993

Kumagai I, Maenaka K, Sunada F, Takeda S, Miura K

Abstract excerpt

The subsite structures in the active site of hen egg-white lysozyme were altered by site-directed mutagenesis. Replacement of Trp62, which is involved in apolar interaction with a sugar ring, and Asp101, which is hydrogen bonded to the same sugar ring in subsite B, led to a shift of the oligosacc...

Topics

Animals
Binding Sites
Chickens
Female
Hydrogen-Ion Concentration
Hydrolysis
Magnetic Resonance Spectroscopy
Muramidase
Mutation
Protein Structure, Secondary
Structure-Activity Relationship

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