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Article

Effects of subsite alterations on substrate-binding mode in the active site of hen egg-white lysozyme.

European journal of biochemistry - 15 Feb 1993

Kumagai I, Maenaka K, Sunada F, Takeda S, Miura K

Abstract excerpt

The subsite structures in the active site of hen egg-white lysozyme were altered by site-directed mutagenesis. Replacement of Trp62, which is involved in apolar interaction with a sugar ring, and Asp101, which is hydrogen bonded to the same sugar ring in subsite B, led to a shift of the oligosaccharide-binding mode in the active-site cleft. Consequently, the double-mutant lysozyme (Trp62His, Asp101Gly) exhibited...

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Identifiers

PMID: 8444153
DOI: 10.1111/j.1432-1033.1993.tb17645.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Effects of subsite alterations on substrate-binding mode in the active site of hen egg-white lysozyme.PMID 8444153Read ↗

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