Article
A new cell culture-based assay quantifies vitamin K 2,3-epoxide reductase complex subunit 1 function and reveals warfarin resistance phenotypes not shown by the dithiothreitol-driven VKOR assay.
Journal of thrombosis and haemostasis : JTH - 1 May 2013
Fregin A, Czogalla K J, Gansler J, Rost S, Taverna M, Watzka M, Bevans C G, Müller C R, Oldenburg J
Abstract excerpt
BACKGROUND: Warfarin directly inhibits the vitamin K 2,3-epoxide reductase complex subunit 1 (VKORC1) enzyme to effect anticoagulation. VKORC1 function has historically been assessed in vitro using a dithiothreitol (DTT)-driven vitamin K 2,3-epoxide reductase (VKOR) assay. Warfarin inhibits wild-type VKORC1 function by the DTT-VKOR assay. However, VKORC1 variants with warfarin resistance-associated missense...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.