Article
Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reaction-high-resolution melting.
Memorias do Instituto Oswaldo Cruz - 1 Feb 2013
Silva Joas Lucas da, Leite Gabriela Guimaraes Sousa, Bastos Gisele Medeiros, Lucas Beatriz Cacciacarro, Shinohara Daniel Keniti, Takinami Joice Sayuri, Miyata Marcelo, Fajardo Cristina Moreno, Luchessi André Ducati, Leite Clarice Queico Fujimura, Cardoso Rosilene Fressatti, Hirata Rosario Dominguez Crespo, Hirata Mario Hiroyuki
Abstract excerpt
Quantitative polymerase chain reaction-high-resolution melting (qPCR-HRM) analysis was used to screen for mutations related to drug resistance in Mycobacterium tuberculosis. We detected the C526T and C531T mutations in the rifampicin resistance-determining region (RRDR) of the rpoB gene with qPCR-HRM using plasmid-based controls. A segment of the RRDR region from M. tuberculosis H37Rv and from strains carrying...
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