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Article

Promotion of markerless deletion of the actinorhodin biosynthetic gene cluster in Streptomyces coelicolor.

Acta biochimica et biophysica Sinica - 1 Oct 2010

Lu Zhiqun, Xie Pengfei, Qin Zhongjun

Abstract excerpt

The standard gene disruption and replacement to delete the actinorhodin biosynthetic gene cluster (Act) in Streptomyces coelicolor was inefficient, and the polymerase chain reaction-targeting of the cosmid could efficiently delete the Act, but still was a time-consuming procedure for markerless gene replacement. By using optimal Streptomyces codons, we synthesized a sceS gene encoding identical amino acid...

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PMID: 20810535
DOI: 10.1093/abbs/gmq080

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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