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A plasmid-based reverse genetics system for influenza A virus

1 Jun 1996

Abstract excerpt

A reverse genetics system for negative-strand RNA viruses was first successfully developed for influenza viruses. This technology involved the transfection of in vitro-reconstituted ribonucleoprotein (RNP) complexes into influenza virus-infected cells. We have now developed a method that allows intracellular reconstitution of RNP complexes from plasmid-based expression vectors. Expression of a viral RNA-like...

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PMID: 8648766
DOI: 10.1128/jvi.70.6.4188-4192.1996

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