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Article

Rescue of Influenza A Virus from Recombinant DNA

1 Nov 1999

Abstract excerpt

We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics...

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Identifiers

PMID: 10516084
DOI: 10.1128/jvi.73.11.9679-9682.1999

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