Electroporation of influenza virus ribonucleoprotein complexes for rescue of the nucleoprotein and matrix genes.

doi:10.1016/0168-1702(95)00031-k

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Article

Electroporation of influenza virus ribonucleoprotein complexes for rescue of the nucleoprotein and matrix genes.

Virus research - 1 Jul 1995

Li S, Xu M, Coelingh K

Abstract excerpt

Reverse genetics has been successfully used for the generation of recombinant influenza virus with altered biological properties. The standard method is based on DEAE-dextran transfection of in vitro reconstituted influenza virus ribonucleoprotein complex (RNP) into helper virus infected cells wi...

Topics

Animals
Base Sequence
Cell Line
DNA Primers
Electroporation
Genes, Viral
Helper Viruses
Humans
Influenza A virus
Molecular Sequence Data
Mutation
Nucleocapsid Proteins
Nucleoproteins
RNA-Binding Proteins
Viral Core Proteins
Viral Matrix Proteins

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