Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Electroporation of influenza virus ribonucleoprotein complexes for rescue of the nucleoprotein and matrix genes.

Virus research - 1 Jul 1995

Li S, Xu M, Coelingh K

Abstract excerpt

Reverse genetics has been successfully used for the generation of recombinant influenza virus with altered biological properties. The standard method is based on DEAE-dextran transfection of in vitro reconstituted influenza virus ribonucleoprotein complex (RNP) into helper virus infected cells with subsequent selection of the recombinant viruses. Here we report the utilization of electroporation for reverse...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Cell Line
Share to DNA Primers

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genes, Viral

Share to Influenza A virus

Share to Molecular Sequence Data

Share to Mutation

Share to RNA-Binding Proteins

Source

Identifiers

PMID: 7483828
DOI: 10.1016/0168-1702(95)00031-k

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Electroporation of influenza virus ribonucleoprotein complexes for rescue of the nucleoprotein and matrix genes.PMID 7483828Read ↗

Engineering influenza viral vectorsSemantic proximity Read ↗

Characterization of influenza virus NS1 protein by using a novel helper-virus-free reverse genetic system.Semantic proximity Read ↗

Introduction of site-specific mutations into the genome of influenza virus.Semantic proximity Read ↗

Reverse Genetics Approaches for the Development of Influenza VaccinesSemantic proximity Read ↗

Transfectant Influenza A Viruses with Long Deletions in the NS1 Protein Grow Efficiently in Vero CellsSemantic proximity Read ↗

A plasmid-based reverse genetics system for influenza A virusSemantic proximity Read ↗

An efficient helper-virus-free method for rescue of recombinant paramyxoviruses and rhadoviruses from a cell line suitable for vaccine development.Semantic proximity Read ↗

Generation of influenza A viruses entirely from cloned cDNAsSemantic proximity Read ↗

Genetic analysis of influenza virus NS1 gene: a temperature-sensitive mutant shows defective formation of virus particles.Semantic proximity Read ↗

Introduction of a temperature-sensitive phenotype into influenza A/WSN/33 virus by altering the basic amino acid domain of influenza virus matrix protein.Semantic proximity Read ↗

Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cellsSemantic proximity Read ↗

Generation of influenza A virus NS2 (NEP) mutants with an altered nuclear export signal sequence.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.