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Article

Generation of influenza A viruses entirely from cloned cDNAs

3 Aug 1999

Abstract excerpt

We describe a new reverse-genetics system that allows one to efficiently generate influenza A viruses entirely from cloned cDNAs. Human embryonic kidney cells (293T) were transfected with eight plasmids, each encoding a viral RNA of the A/WSN/33 (H1N1) or A/PR/8/34 (H1N1) virus, flanked by the human RNA polymerase I promoter and the mouse RNA polymerase I terminator-together with plasmids encoding viral...

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Identifiers

PMID: 10430945
DOI: 10.1073/pnas.96.16.9345

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