Article
Identification of the vaccinia virus gene encoding an 18-kilodalton subunit of RNA polymerase and demonstration of a 5' poly(A) leader on its early transcript.
Journal of virology - 1 Jun 1990
Ahn B Y, Jones E V, Moss B
Abstract excerpt
The DNA-dependent RNA polymerase of vaccinia virus contains 8 to 10 virus-encoded polypeptides. We have mapped the gene encoding an 18-kilodalton RNA polymerase subunit to D7R, the seventh open reading frame of the HindIII D genomic subfragment. Localization of this gene was achieved by using ant...
Topics
- Base Sequence
- DNA-Directed RNA Polymerases
- Genes, Viral
- HeLa Cells
- Humans
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Poly A
- Promoter Regions, Genetic
- Protein Biosynthesis
- RNA, Viral
- Restriction Mapping
- Temperature
- Transcription, Genetic
- Vaccinia virus
- Viral Structural Proteins
